Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration Research Published on 2022-05-132022-10-05 Journal: Signal Transduction and Targeted Therapy [Category] 바이오마커, [키워드] abnormality addressed ameliorated Analysis approach attenuate biological processe chemotaxis chronic inflammation develop disease drug Drug discovery effective EGFR fibrosis gefitinib immune cell recruitment Inflammation inhibit inhibited macrophage activation mechanism Pathogenesis phosphoproteome Phosphorylation potential therapeutic target prevalent progression Protein proteome provide pulmonary dysfunction signaling pathway significant difference silicosis SYK target targets tested the disease therapeutic strategy transcript Transcriptome Treatment [DOI] 10.1038/s41392-022-00959-3 [Article Type] Research
Dysregulation of the leukocyte signaling landscape during acute COVID-19급성 COVID-19 동안 백혈구 신호 전달 환경의 조절 장애Article Published on 2022-04-142022-09-12 Journal: PLoS ONE [Category] 진단, [키워드] Activation acute COVID-19 approach Cell cellular activation circulating claimed correlated COVID-19 COVID-19 pandemic Cytometry driving dysregulated functional IFN-γ IL-6 level immune dysfunction immune effector cell immune function immune system immunology Immunosuppression Inflammation leukocyte lung lymphoid marker mechanism molecular mechanism monocyte myeloid cell pathogenesis of COVID-19 pathophysiologic phosphoproteome Phosphorylation populations Signaling signaling pathway STAT3 suppressed systemic inflammatory response T cell T cells These data tissue injury [DOI] 10.1371/journal.pone.0264979 PMC 바로가기 [Article Type] Article
The Candida albicans Cdk8-dependent phosphoproteome reveals repression of hyphal growth through a Flo8-dependent pathway칸디다 알비 칸스 CDK8- 의존적 포스 포 단백체는 FLO8- 의존적 경로를 통한 균사 성장의 억압을 나타낸다.Research Article Published on 2022-01-042022-09-06 Journal: PLoS Genetics [Category] Communicable Disease, [키워드] acquisition activities Affect Amino acid amino acids analyses ATP Candida Candida albican Candida albicans Cdk8 cell interactions cellular conditions dead dependent on drug resistance functional growth hypha hyphal identify Immune cell Impact inactivation increased expression inhibited Interaction interactions involved leads mediator Mediator complex medium member metabolism modulate Morphological morphological changes morphology mutant Mutation nutrient pathway performed phenotype Phenotypes phosphoproteome phosphorylation site physiological promoted Protein Protein phosphorylation sites Rapid reduced regulate Regulation regulator replaced reveal Serine shown Steady state strain substrate target targets the strongest These data threonine Transcription transcription factor transcriptional transcriptomics variant virulence was used [DOI] 10.1371/journal.pgen.1009622 PMC 바로가기 [Article Type] Research Article
Proteomic Signature of Host Response to SARS-CoV-2 Infection in the Nasopharynx비인두에서 SARS-CoV-2 감염에 대한 숙주 반응의 단백질 서명Research Published on 2021-08-142022-09-10 Journal: Molecular & Cellular Proteomics : MCP [Category] 진단, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addition AGC, automatic gain control airway AKT Analysis antiviral property antiviral signaling asymptomatic infection caused CEP131 CFAP57 characterized ciliary component components confirmed COVID-19 case controls coronavirus coronavirus disease Coronavirus disease 2019 Coronavirus infections COVID-19 COVID-19 severity COVID-19, Coronavirus disease 2019 CXCL10 downregulation dysregulated immune response dysregulation E3 ubiquitin ligases FDR, false discovery rate Health High-resolution host response humans IFN, interferon immune response implicated infected individual infected individuals Infection Inflammatory response interferon interferon-stimulated gene interferon-stimulated genes investigator investigators involved ISG15 ISGs, interferon-stimulated genes mechanism MERS-CoV, Middle East respiratory syndrome coronavirus molecular MPB, mobile phase B multiorgan disease MX1 nasopharyngeal swabs negative control negative controls NHBE, normal human bronchial epithelial novel NP, nasopharyngeal OGFR, opioid growth factor receptor OPRPN, Opiorphin prepropeptide pandemic Pathogenesis pathogenesis of COVID-19 Pathways performed phosphoproteome Phosphoproteomics Phosphorylation PKC PPIase, peptidyl-prolyl cis/trans isomerase PRM, parallel reaction monitoring proteasomal Protein protein synthesis proteomic Proteomic analysis proteomics PRRs, pathogen-recognition receptors respiratory response RIG-I SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome Coronavirus shown signaling pathway signature Stat1 subunits Swab These data upper airway upregulated upregulation valuable resource Viral viral entry viral infection viral infections virus [DOI] 10.1016/j.mcpro.2021.100134 PMC 바로가기 [Article Type] Research
Kinetic Multi-omic Analysis of Responses to SARS-CoV-2 Infection in a Model of Severe COVID-19중증 COVID-19 모델에서 SARS-CoV-2 감염에 대한 반응의 동역학적 다중 오믹 분석Pathogenesis and Immunity Published on 2021-07-282022-09-10 Journal: Journal of Virology [Category] SARS, 유전자 메커니즘, 진단, 치료기술, [키워드] absence active viral infection acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 alveolar animal model animal models approach caused changes in chemokines coronavirus coronavirus disease Coronavirus disease 2019 Course COVID-19 develop disease effort evaluated Extrapulmonary gene transcription hamster heart host response host responses human disease human isolates of SARS-CoV-2 immune in vivo increase in Infection innate immune response interferon interferon-stimulated gene isolate kidney kidneys kinetic kinetic analysis lack Model Monocytes Multiorgan neutrophil pandemic Pathogenesis pathology peaked phosphoproteome Phosphoproteomics Pneumonia protein expression proteome proteomics pulmonary disease respiratory response RNA-Seq SARS-CoV-2 SARS-COV-2 infection severe acute respiratory syndrome Coronavirus severe coronavirus disease severe COVID-19 severe SARS severe SARS-CoV-2 These data to define Transcription Transcriptome transcriptome changes type I Viral viral infection [DOI] 10.1128/JVI.01010-21 PMC 바로가기 [Article Type] Pathogenesis and Immunity
Mass spectrometry-based proteomic platforms for better understanding of SARS-CoV-2 induced pathogenesis and potential diagnostic approachesSARS-CoV-2 유도 병인 및 잠재적 진단 접근법에 대한 더 나은 이해를 위한 질량 분석 기반 단백질체 플랫폼Review Published on 2021-05-012022-09-11 Journal: Proteomics [Category] SARS, 유전자 메커니즘, 진단, [키워드] AMPK applied approach Biomarkers cellular Comparative analysis comparative proteomics COVID-19 dataset diagnostic EGFR EPHA2 Ephrin functional humans in vitro experiments indicate Infection kinase kinase-substrate signaling kinases knowledge MAPK mass mass spectrometry mechanism molecular mechanism molecular mechanisms Pathogenesis pathway Pathways phosphoproteome PI3K platform post-translational modifications prognostic Protein protein targets protein-protein interaction proteome proteomic proteomics receptor SARS-CoV-2 SARS-COV-2 infection signaling pathways targeted proteomics the SARS-CoV-2 Therapeutic strategies tissue tissue sample tissue samples top-down proteomics. Viral viral infection while [DOI] 10.1002/pmic.202000279 PMC 바로가기 [Article Type] Review
Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV Research Published on 2021-04-122022-10-05 Journal: Nature [Category] SARS, 신약개발, 치료제, [키워드] Antiviral effect autophagy both viruses cellular interaction Coronaviruses COVID-19 dataset drug dysregulated fibrosis heterogeneity highlight Host Host-directed therapies hotspot human cell infection with SARS-CoV-2 inhibitor integration Interaction kinase knowledge matrix metalloprotease molecular function molecular mechanism ORF3 ORF8 pathogenic pathway Perturbation phosphoproteome proteome proteomics reveal SARS-CoV SARS-CoV-2 Spread TGF-β These data tissue to define Transcriptome Viral protein virus virus–host interaction [DOI] 10.1038/s41586-021-03493-4 [Article Type] Research
SARS-CoV-2 vaccine ChAdOx1 nCoV-19 infection of human cell lines reveals low levels of viral backbone gene transcription alongside very high levels of SARS-CoV-2 S glycoprotein gene transcriptionSARS-CoV-2 백신 ChAdOx1 nCoV-19 인간 세포주의 감염은 매우 높은 수준의 SARS-CoV-2 S 당단백질 유전자 전사와 함께 낮은 수준의 바이러스 백본 유전자 전사를 나타냅니다Research Published on 2021-03-152022-08-13 Journal: Genome Medicine [Category] SARS, 유전자 메커니즘, 치료기술, [키워드] A549 A549 cell A549 cell line A549 cell lines A549 cells addition Adenovirus approach backbone Cell Cell culture cell line cell lines Cell Nucleus cells ChAdOx1 ChAdOx1 nCoV-19 ChAdOx1 nCoV-19 vaccine clinical trial coding Direct RNA sequencing expected expression Gene Expression Genome globe HEK293 human cell line Infection nucleus phosphoproteome polyadenylation site Protein proteome proteomic proteomics Quantitative recombinant adenovirus Replication Result reveal S glycoprotein SARS-CoV-2 SARS-CoV-2 S SARS-CoV-2 S glycoprotein SARS-CoV-2 vaccine splice supplementary material the cell the SARS-CoV-2 transcript Transcription transcriptomic Vaccine Viral [DOI] 10.1186/s13073-021-00859-1 PMC 바로가기 [Article Type] Research
Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoproteinSARS-CoV-2의 전사체 및 프로테옴의 특성화는 스파이크 당단백질에서 푸린 유사 절단 부위의 세포 통과 유도된 프레임 내 결실을 보여줍니다Research Published on 2020-07-282022-08-13 Journal: Genome Medicine [Category] SARS, 유전자 메커니즘, [키워드] 24 nt in-frame deletion analysed Analysis animal challenge model animal challenge models Cell cleavage clinical samples contribute Coronaviruses deleted detection determine Direct RNA sequencing exit expected Frame functional furin furin cleavage site furin site furin-like cleavage site genomic glycoprotein growth Health impacted in-frame deletion infected cells mass spectrometry morbidity morbidity and mortality mutate Novel coronavirus ORF-centric pipeline Pathogenesis peptide peptides phosphopeptide phosphoproteome predicted Protein proteome proteomic Region Research respiratory respiratory pathogen Result reveal S glycoprotein SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 protein SARS-CoV-2 proteins significantly spike glycoprotein subgenomic mRNA subgenomic mRNAs subunit tandem Tandem mass spectrometry the SARS-CoV-2 genome Transcription Transcriptome transcriptomic unique Vaccine variant Variation Vero E6 Vero E6 cell VERO E6 cells viable deletion Viral viral genome sequence viral peptide viral peptides viral stock viral stocks viral transcript viral transcriptome viral transcripts virulence virus virus entry virus-infected cells zoonosis [DOI] 10.1186/s13073-020-00763-0 PMC 바로가기 [Article Type] Research
The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suisOriginal Research Published on 2017-03-072023-05-31 Journal: Frontiers in Cellular and Infection Microbiology [Category] Fulltext, 조류인플루엔자, [키워드] Streptococcus suis eukaryote-like serine/threonine kinase growth metabolism phosphoproteome Phosphorylation RNA-Seq virulence [DOI] 10.3389/fcimb.2017.00066 PMC 바로가기 [Article Type] Original Research